A Guide to the Meso-Scale Production of the Copepod Acartia tonsa

Abbreviations
Suppliers

Constructing the Squeegee Siphon Assembly

The squeegee is designed to gently scrape the bottom of the tank to retrieve the eggs produced since the last harvest. A siphon hose alone will not capture all eggs entrapped in the biofilm that builds up in living systems. The tool needs to conform gently to the bottom of the tank without damaging the Fiberglas gelcoat. Plumbing diameters need to be sized to maintain siphon velocity and flow without overwhelming the stacked sieves. Many alternate strategies could work. Our solution was to construct a simple siphon from PVC and install a section of Shop-Vac squeegee (an accessory for the wet vacuum head) as a scraper (Figures 8, 9, 10).

For a 300 L tank, construct a ¾” (1.9 cm) I.D. PVC siphon assembly:

¾” diameter PVC pipe, at least 60” long

¾” PVC tee fitting

¾” PVC couplings

¾” PVC 45° elbow

¾” PVC female fitting

¾” PVC male to hose barb adapter

7’ (2 m) section siphon hose

¾” Hose clamp for siphon hose

6”- 9” (15-23 cm) section of Shop-Vac squeegee

PVC discs cut from 4” PVC test caps

Large report-style paper clamp

Profile View of Squeege Head on View of Squeegee

Figure 8. Squeegee Scraper

1.Glue the PVC tee and couplings together with sections of pipe to fill the interior voids as completely as possible. 6”- 9” (15-23 cm) siphon head width is the optimum width for this design.

2. Use a Dremel tool or hack saw to slice a slot opposite the tee inlet, cutting through both the fittings and the PVC pipe glued inside. The slot’s interior faces are cut parallel, not splayed apart. The slot’s width matches the width of the Shop-Vac squeegee, measured under the outer flange. Trim a section of squeegee to fit and cement in place with silicone aquarium sealant.

Use of the Squeegee Siphon

Side View of Squeegee

Figure 9. Squeegee cross-section schematic.

Squeegee Constructuction Diagram

Figure 10. Squeegee longitudinal section schematic.